Effects of gallic acid on the cell structure of Xanthomonas oryzae pv. oryzicola

分类： 生物学 >> 植物学 >> 应用植物学 提交时间： 2018-10-26 合作期刊: 《广西植物》

ZHANG Xijiao LI Fangjing YUAN Gaoqing WEI Changying LIN Wei LI Qiqin

摘要： Gallic acid (GA) is a phenolic compound, and presents various biological activities in plants. Our previous experiments demonstrated a relatively strong inhibitory effect of GA on Xanthomonas oryzae pv. oryzicola (Xoc). In order to elucidate the effects of GA on the cell structure and membrane permeability of the pathogen, morphologies of bacteria treated with GA were observed by electron microscopy, the integrity and the permeabilities of membrane of Xoc were investigated by determining the release from cells of materials that absorb at 260 nm, changes in the fluorescence of cells treated with thefluorescein diacetate(FDA) and lactate dehydrogenase(LDH) activity. Treatment with 200 μg·mL-1, many pits and irregular vesicles were observed on the cell surface under electron microscopy, indicating that GA could damage the cell walls of Xoc. The electrical conductivity from Xoc suspensions 24 h after GA treatment was 135.48 μS·cm-1（Control was 127.85 μS·cm-1）, and the fluorescence intensity of Xoc suspensions 2 h after GA decreased by 58.10%, indicating that cells leaked electrolytes and cytosolic contents. Meanwhile, the activities of LDH in bacterial suspensions treated with GA also increased, suggesting that GA could damage the structure of the bacterial cell membrane. In addition, the absorbance at 260 nm from Xoc suspensions was 1.004（control was 0.018）, indicating that GA could negatively affect Xoc cell wall integrity. These results indicate that GA not only altered the permeability of the cell membrane of Xoc, but also impacted the integrity of the cell membrane.

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